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KMID : 0355820190400010051
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Korean Journal of Oral Anatomy
2019 Volume.40 No. 1 p.51 ~ p.59
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Shikonin inhibits the Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma
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Park Dan-Bi
Park Bong-Soo
Kang Hae-Mi
Yu Su-Bin
Kim In-Ryoung
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Abstract
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Oral cancer invades the surrounding muscles and bone tissue and metastasizes to the cervical lymph nodes. The 5-year survival rate of patients with metastases is about 40%, and it is necessary to develop anticancer drugs that suppress metastasis to improve the prognosis of patients. In epithelial-mesenchymal transition (EMT), the squamous cancer cells have mobility, which increases the invasiveness and metastasis to new organs. Therefore, EMT inhibition is considered a therapeutic method for inhibiting the metastasis of cancer cells. Shikonin has been isolated and purified from Lithospermum erythrorhizon Sieb. et Zucc and is widely known for its anti-inflammatory effect. Recently, shikonin has been shown to induce apoptosis and cell cycle arrest in various cancer cells and to inhibit the metastatic properties of lung and gastric cancer cells. However, the effects of shikonin on oral cancer are still unclear. Therefore, we investigated the effect of EMT inhibition on shikonin-treated oral cancer cell line, CAL-27 cells. Shikonin treatment inhibited the viability and proliferation of CAL-27 cells. In addition, shikonin inhibited their migration and invasion in wound-healing and transwell invasion assays. Shikonin increased the protein expression of E-cadherin, an epithelial marker in CAL-27 cells, and decreased the protein and RNA expression of mesenchymal markers like N-cadherin, Snail, and Slug. Therefore, shikonin inhibited the epithelial-mesenchymal metastasis of oral cancer cell line CAL-27, suggesting that it may be an anticancer agent with an anti-metastatic effect. 줄이기
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KEYWORD
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Shikonin, EMT, migration, invasion, OSCC
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